The most common and most likely answer to this crossword clue is the 13 letter word INTERROGATION
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More clues leading to the result INTERROGATION
Buddy explains!

The straight part
We know that "Questioning" is the straight/definition part of the clue. The straight part is often a synonym or definition, in this case questioning = interrogation.The cryptic/wordplay part
In cryptic crossword there's often an wordplay/cryptic part as well.This part involves a more complex hint, such as an anagram, homophone, hidden word, etc.
Sadly, We don't have an explanation for this specific crossword clue yet.
What does INTERROGATION mean?
INTERROGATION noun- The act of interrogating or questioning; examination by questions; inquiry.
- A question put; an inquiry.
- A question mark.