The most common and most likely answer to this crossword clue is the 7 letter word STEEPEN
I've seen this crossword clue in The Guardian
S
T
E
E
P
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More clues leading to the result STEEPEN
Buddy explains!

The straight part
We know that "Toughen up" is the straight/definition part of the clue. The straight part is often a synonym or definition, in this case toughen up = steepen.The cryptic/wordplay part
In cryptic crossword there's often an wordplay/cryptic part as well.This part involves a more complex hint, such as an anagram, homophone, hidden word, etc.
Sadly, We don't have an explanation for this specific crossword clue yet.
What does STEEPEN mean?
STEEPEN verb- To make steeper.
- To become steeper.