Cryptic crossword clue
Tries once more removing Bottom from what actors do with play

The most common and most likely answer to this crossword clue is the 7 letter word REHEARS

R
E
H
E
A
R
S

Buddy explains!

The straight part
We know that "Tries once more" is the straight/definition part of the clue. The straight part is often a synonym or definition, in this case tries once more = rehears.
The cryptic/wordplay part
In cryptic crossword there's often an wordplay/cryptic part as well.
This part involves a more complex hint, such as an anagram, homophone, hidden word, etc.
Sadly, We don't have an explanation for this specific crossword clue yet.

What does REHEARS mean?

REHEARS verb
  1. To hear again.
  2. To try (a lawsuit, etc.) again judicially.

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